The objective of this work was to examine the effect of solution pH and ionic strength on virus retention by the Viresolve(®) NFP membrane. Data were obtained using the bacteriophage ϕX174 as a model virus, with retention data complemented by the use of confocal microscopy to directly visualize capture of fluorescently labeled ϕX174 within the filter.

Viresolve NFR FiltersClear Sorting & Filtering Show Filter Hide Filter; Catalog Number Description Cartridge Nominal Length Process Volume Cartridge Code Pack Size; CZRV71TP1: Viresolve NFR Cartridge Filter 10 in. Code 7 10 in. (25 cm) 600 L–1200 L Code 7 1 Show Pricing & Availability

2014-05-15 · These were Optiscale-25 devices with Viresolve NFP membrane (3.5 cm 2 surface area Millipore), Virosart CPV filter discs (5.3 cm 2 surface area; Sartorius), Ultipor VF grade DV20 filter discs in a re-usable stainless-steel housing unit (Pall housing part number FTK200; 11.1 cm 2 surface area Pall Corp.), and Planova 20N small-scale hollow-fiber devices (10.0 cm 2 surface area Asahi Kasei). Viresolve NFP (normal flow parvovirus) membranes from Millipore are high-flux composite membranes that use a size-exclusion mechanism to achieve a greater than four-log removal of parvoviruses. The Viresolve NFP filtration step was a robust and effective step in removing all viruses tested, since HIV, BHV, BVDV, HAV, EMCV, and PPV were completely removed during the filtration step with log reduction factors of ≥ 5.51, ≥ 5.76, ≥ 5.18, ≥ 5.34, ≥ 6.13, and ≥ 4.28, respectively. Results for the Viresolve® Pro membranes showed much greater pore interconnectivity than for the Viresolve® NFP membranes. SEM images of the Ultipor® DV20 membrane showed nanoparticle capture only at regions of the inlet located directly over the open portions of the membrane exit, indicating that there is minimal lateral flow in this membrane.

Viresolve nfp

Such filters are designed to be used in normal flow mode (dead-end mode) and can be operated at constant pressure or constant flow. Optiscale-40 devices with Viresolve prefilters (5.0 cm 2) were also purchased. The objective of this work was to examine the effect of solution pH and ionic strength on virus retention by the Viresolve ® NFP membrane. Data were obtained using the bacteriophage ϕ{symbol}X174 as a model virus, with retention data complemented by the use of confocal microscopy to directly visualize capture of fluorescently labeled ϕ{symbol}X174 within the filter.

Operated in normal flow filtration mode, these filters are ideal for monoclonal antibody polishing applications eliminating small virus contaminants from essential media and feed streams containing dilute peptides. Opticap XL and XLT Disposable Capsule Filters with Viresolve NFP Membrane Viresolve NFP filters with Viresolve NFP (Normal Flow Parvovirus) membranes clear parvovirus from recombinant or human plasma sources, without compromising flow rates.

This product was manufactured with a Viresolve ® membrane which meets the criteria for a "non-fiber releasing" filter as defined in 21 CFR 210.3 (b) (6). Legal Information VIRESOLVE is a registered trademark of Merck KGaA, Darmstadt, Germany

. Lab Results for the Viresolve® Pro membranes showed much greater pore interconnectivity than for the Viresolve® NFP membranes.

to compress multiple stages of single-layer OptiScale-25 capsules with Viresolve NFR filtration into a single element. Using filters or NFP membranes are used

Merck’s product portfolio includes a vast range of filters and single-use systems, comprising Viresolve Prefilter Pod Disposable Devices, Viresolve NFP Cartridge Filters etc. Over the past three years, the company adopted the strategy of new product launches and acquisitions to maintain its leadership position in the global virus filtration market. 2010-01-14 2014-09-07 Description. Viresolve NFP filters with Viresolve NFP (Normal Flow Parvovirus) membranes clear parvovirus from recombinant or human plasma sources, without compromising flow rates. Viresolve NFP filters add speed and dependability to viral clearance in therapeutic drug manufacturing applications. Viresolve NFP filters with Viresolve NFP (Normal Flow Parvovirus) membranes clear parvovirus from recombinant or human plasma sources, without compromising flow rates. - Find MSDS or SDS, a COA, data sheets and more information.

Operated in normal flow filtration mode, these Viresolve NFP Cartridge Filter 20 in. Code 7 Parvovirus removal; find Millipore-CVPV72TP1 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
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Optiscale-40 devices with Viresolve prefilters (5.0 cm 2) were also purchased. Results for the Viresolve® Pro membranes showed much greater pore interconnectivity than for the Viresolve® NFP membranes. SEM images of the Ultipor® DV20 membrane showed nanoparticle capture only at regions of the inlet located directly over the open portions of the membrane exit, indicating that there is minimal lateral flow in this membrane. Viresolve NFP (normal flow parvovirus) membranes from Millipore are high-flux composite membranes that use a size-exclusion mechanism to achieve a greater than four-log removal of parvoviruses.

The objective of this work was to examine the effect of solution pH and ionic strength on virus retention by the Viresolve ® NFP membrane. Data were obtained using the bacteriophage ϕX174 as a model virus, with retention data complemented by the use of confocal microscopy to directly visualize capture of fluorescently labeled ϕX174 within the filter.
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Water wet filter for 5 min @ 3.4 bar (50 psi); liquid cycle, slow exhaust @ 123 °C maximum 60 min. This product was manufactured with a Viresolve ® membrane which meets the criteria for a "non-fiber releasing" filter as defined in 21 CFR 210.3 (b) (6).

Code 7 Parvovirus removal - Find MSDS or SDS, a COA, data sheets and more information. Viresolve NFP filters with Viresolve NFP (Normal Flow Parvovirus) membranes clear parvovirus from recombinant or human plasma sources, without compromising flow rates.

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Search for more papers by this author. Water wet filter for 5 min @ 3.4 bar (50 psi); liquid cycle, slow exhaust @ 123 °C maximum 60 min. This product was manufactured with a Viresolve ® membrane which meets the criteria for a "non-fiber releasing" filter as defined in 21 CFR 210.3 (b) (6). The objective of this work was to examine the effect of solution pH and ionic strength on virus retention by the Viresolve(®) NFP membrane. Data were obtained using the bacteriophage ϕX174 as a model virus, with retention data complemented by the use of confocal microscopy to directly visualize capture of fluorescently labeled ϕX174 within the filter.