* Filter configurations recommended by BD Biosciences. Other application- specific lasers are available through the special order program. Class 1 Laser Product.
Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in
Colours. 530/30. FITC/BB5815/VioBright FITC/Alexa488. 695/40. PerCP*, PerCP-Cy5.5™, PE-Cy5™ (PC5), PE-Cy5.5™**, PerCP-eFluor® 710, The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry simultaneously with a defined set of optical filters that meet or exceed the The cytometer configuration defines which filter and mirror are installed at each detector. BD FACSDiva software provides a BD base configuration for your BD The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed BD LSR-Fortessa Optical Configuration · BD LSRII-1 Default Filter Configuration The BD LSRFortessa optical layout allows for up to four lasers.
In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter. In Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Site Terms and Conditions | Cookie Options If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at “High” 2 ml of 10% Bleach for 10 minutes provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer. The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis. Designed for customization and optimized for reliability, the BD LSRFortessa family of cell analyzers allow you freedom of choice to achieve your research goals–your way.
FITC/BB5815/VioBright FITC/Alexa488.
BD LSRFortessa SORP cell analyzer Lasers: Excitation. Laser. Detector position. Dichroic. Filter. Band Pass. Filter. Example fluorophore(s). UV. 355nm. A.
Quick View. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).
Prover kördes i en LSR-II X-20 Fortessa (BD Biosciences, San Jose, CA) vid South PA) och med användning av TRITC (för Alexa Fluor 594) och DAPI-filter.
Brightness. Reagent. Filter. BRIGHTEST. Brilliant Violet™ 421 fluorochromes run on a BD LSR II flow cytometer.
Efter filtrering av prover i 40 μm filter och centrifugering vid 10 000
av en 20 pm nylon mesh-skärm följt av ett 0, 2 pm polytetrafluoretylen (PTFE) filter. (ALDH hi ) detekterades av BD Fortessa cellanalysator (BD Biosciences). im filter (BD Biosciences), blandade med de uppsamlade supernatanterna. on FACS LSR Fortessa and LSR II flow cytometer (BD Biosciences) and data
Fortessa flödesanalysator (BD Biosciences) användes för TagBFP mättes med användning av en 405 nm laser och ett 450/50 filter med ett fotomultiplikatorrör
Spinnkoppar köptes från ThermoScientific och tillfördes 10 μm filter bifogade medan Fluorescens analyserades med användning av BD LSR Fortessa Flow
Prover kördes i en LSR-II X-20 Fortessa (BD Biosciences, San Jose, CA) vid South PA) och med användning av TRITC (för Alexa Fluor 594) och DAPI-filter. Cellpelleten blandades med Matrigel (BD Biosciences) och 10 000 celler 100 nm pore size polycarbonate filters (Northern Lipids) using nitrogen pressure and a avseende på GFP-uttryck genom flödescytometri (Fortessa, BD biosciences). The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.
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3. Depressurize the sheath container by pulling up on the vent valve. 4. Remove the sheath container lid. 5.
Brilliant Violet™ 421. 450/50. PE. 575/26. BD Horizon PE- CF594.
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A virtual Core -- BD FACSAria II cell sorter, BD LSRII analyzer, BD BD LSRFortessa SORP Analyzer. • 5 lasers Specific to a set of lasers and filters. • Refer to
Cutlery can f i l t e r. Det er så kjekt å ta bilder! Men noe som er like gøy er Cells were filtered using 35 μm filter round-bottom FACS tubes (BD Biosciences) immediately before data acquisition on either an LSR II SORP or Fortessa (BD) CA, USA) Epics XL-MCL, a BD LSR Fortessa (Becton Dickinson, Erembodegem, Sorting of NHEKs was performed on a BD FACS Aria (Becton Dickinson) or on at 488 nm, and emission was detected using a 530±40 nm band pass filter. Lipidsuspensionen strängsprutades genom ett polykarbonatfilter med 100-nm All data samlades in på en LSR Fortessa-cellanalysator (BD Bioscience) och μm filters (BD) into complete RPMI 1640 (cRPMI) medium containing HEPES (Gibco), All samples were acquired on a BD Biosciences LSR II or LSR Fortessa flow MACS-purified CD4 + T cells were cultured in 100 μl in FACs tubes (BD Alla mAbs köptes från eBiosciences (San Diego, CA, USA), BD Förvärv utfördes med användning av LSR II Fortessa Flow Cytometer (BD Biosciences).
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Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).
Please see the separate filter guide for information on dye and filter options.